Fig. 6. Time course of Rb⁺ uptake, [K]_i, and Hb_c ODs in CR AgNPs-treated RBCs from human cord blood. Cord blood donated by MVH was subjected to the procedures described in Materials and Methods and Fig. 1. During the flux period, cells were incubated at 37°C for 15, 30, and 60 min under the conditions described in legend to Fig. 3. A) Rb⁺ uptake, B) [K]_i and C) Hb_c ODs at 527 nm for Rb⁺ uptake and [K]_i. Values are mean ± S.D. for a representative experiment, n= 6 individual determinations for all the conditions tested. The statistical significance of the differences between NPS60 vs NPS15 or NPS30 and NPS60 vs CONT60 was determined by paired t- test. Further details are discussed in the text. A) *** and # p = 0.0000 for NPS15 vs NPS60 and NPS60 vs CONT60, respectively; * p<0.05 for NPS30 vs NPS60. B) *** and # p = 0.0000 for NPS15 vs NPS60, NPS30 vs NPS60, and NPS60 vs CONT60, respectively; and C) # p = 0.0001 forNPS60 and CONT60.